ABSTRACT
The aim of this study is to evaluate the efficacy of total saponins of Dioscorea (TSD), an extract of the Chinese herbal Bi Xie, on hyperuricemia and to elucidate the underlying mechanisms. The rat hyperuricemia model was established by administration of adenine. Thirty-two rats were randomly allocated into 4 groups: model group, low/high-dose TSD-treated groups, and allopurinol-treated group. Meanwhile, 8 rats were used as normal controls. Serum uric acid (UA), blood urea nitrogen (BUN), serum creatinine (Scr), and organic anion transporting polypeptide 1A1 (OATP1A1) levels were measured. Comparison between the model group and treatment (allopurinol and TSD) groups showed the serum UA levels were significantly decreased in treatment groups. TSD had similar effects to allopurinol. It was found that the OATP1A1 protein expression levels in treatment groups were higher than in model group and normal controls. And different from the allopurinol-treated groups, TSD-treated group had elevated OATP1A1 expression levels in the stomach, liver, small intestine and large intestine tissues. It was suggested that TSD may facilitate the excretion of UA and lower UA levels by up-regulating OATP1A1 expression.
Subject(s)
Animals , Male , Rats , Creatinine , Blood , Dioscorea , Chemistry , Drugs, Chinese Herbal , Pharmacology , Therapeutic Uses , Hyperuricemia , Drug Therapy , Intestines , Metabolism , Liver , Metabolism , Organic Anion Transporters, Sodium-Independent , Genetics , Metabolism , Rats, Sprague-Dawley , Saponins , Pharmacology , Therapeutic Uses , Stomach , Metabolism , Up-Regulation , Uric Acid , BloodABSTRACT
Objective To study the chemical constituents of Dioscorea septemloba.Methods The compounds were isolated by GC-MS and chromatography on silica gel column and identified on the basis of physico-chemical constants and spectral analysis.Results Thirteen compounds were isolated and their structures were identified as 4,8-dimethyl-1,7-nonadiene(Ⅰ),5-isopropyl-2,8-dimethylcyclodeca(Ⅱ),3,7-dimethyl-6-octen-1-ol-formate(Ⅲ),dodecanoic acid methyl easter(Ⅳ),N,N'-dinitro-1,2 cyclohexanddiamine(Ⅴ),1-octyn-4-ol(Ⅵ),?-sitosterol(Ⅶ),palmitic acid(Ⅷ),stigmasterol(Ⅸ),6,7-dihydroxy-2-methoxy-1,4-phenanthrenedione(Ⅹ),diosgenin(Ⅺ),ruscogenin(ⅩⅡ),and stigmasterol-3-O-?-D-glucoside(ⅩⅢ).Conclusion Compounds Ⅰ—Ⅵ,Ⅸ,Ⅹ,ⅩⅡ,and ⅩⅢ are obtained from this plant for the first time.